The Isolated Perfused Rat Kidney: A Technical Update
نویسندگان
چکیده
منابع مشابه
The Isolated Perfused Rat Kidney
1. In the isolated rat kidney, perfused at constant pressure with a medium free from renin substrate, addition of angiotensin I to the perfusate decreases renal 'plasma' flow. 2. A peptide inhibitor, SQ 20881, of converting enzyme reduces the vasoconstrictor effect of angiotensin I up to a maximum of 87%, the degree of inhibition being dose-dependent. 3. The molar ratio of equi-effective doses ...
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The perfused rat kidney was used for a study of gluconeogenesis. The kidney was completely isolated and perfused with Krebs-Henseleit bicarbonate buffer containing 5% albumin. Perfusion flow rate and pressure remained stable for 2 to 3 hours, although the glomerular filtration rate diminished with time. The fractional reabsorption of both water and sodium was 0.92 during the 1st hour of perfusi...
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1. Renal mechanisms of conjugated bilirubin excretion have been studied in isolated rat kidneys perfused with a protein-free dextran medium, containing conjugated bilirubin isolated from human bile. 2. In nine perfused kidneys with a low glomerular filtration rate (GFR) (less than 0.5 ml/min) and depressed tubular function, there was a significant linear correlation between conjugated bilirubin...
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Nephrotoxicity is an important limitation to the clinical use of colistin against Pseudomonas aeruginosa and other gram-negative pathogens. Previous work reported net tubular reabsorption of colistin by the kidney in vivo, but there is no knowledge of its disposition within the kidney. This study investigated the renal disposition and potential transport mechanisms of colistin in the isolated p...
متن کاملCharacterization of pentamidine excretion in the isolated perfused rat kidney.
OBJECTIVE To study the renal excretion and kidney accumulation of pentamidine, a potentially nephrotoxic compound, in the isolated perfused rat kidney (IPK). MATERIALS AND METHODS IPK experiments (3-4 per treatment group) were conducted using male Sprague-Dawley rats (250-350 g). Dose proportionality studies were carried out over a pentamidine dosing range of 80-4000 microg, designed to targe...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 2013
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.62.19